The present book comprises twelve chapters and comprehensive appendices. The first five chapters contain the directions and procedural details for the estimation of carbohydrates, proteins, amino acids, phenols, lipids and enzymes. The assays for enzymatic and non-enzymatic antioxidants has mentioned in details in chapter six. The chapter seven, eight and nine deals with the extraction and quantifications procedures of nucleic acids, pigments and plant growth regulators, respectively. The author has covered in chapter ten about the analysis of macro- and micro-nutrients from plant samples in chapter ten. The chapter eleven is exclusively discussed about the microscopic techniques for plant tissue analysis which is foremost imperative for histological study. And the last chapter deals with respective formulae need for the growth analysis parameters. The book has concluded with more useful appendices. This book helps in understanding the different phytochemical analytical methods with step-by-step approach to virtually every topic pertaining in plant analysis with extremely comprehensive scope. This book is unique compilation which cover various plant analytical methods under a single domain. Hope that this book will fulfil the demand of researcher, students as well as teachers with a great standard.